Review



pretreatment with tg  (TargetMol)


Bioz Verified Symbol TargetMol is a verified supplier
Bioz Manufacturer Symbol TargetMol manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    TargetMol pretreatment with tg
    Pretreatment With Tg, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pretreatment+with+tg/Thapsigargin/pm39966508-51-32-41
    Average 94 stars, based on 18 article reviews
    pretreatment with tg - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Knockdown:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Expressing:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Western Blot:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Quantitative RT-PCR:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Control:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Inhibition:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Over Expression:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Immunofluorescence:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.

    Activation Assay:

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO2. .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment. .. Construction of XBP1s knockdown cell lines For knockdown of XBP1s (GenBank accession number: XM 018061044.1) in gEECs, we designed three pairs of olig

    Article Title: ERS regulates endometrial epithelial cell autophagy through XBP1s -mediated activation of the PI3K/AKT pathway
    Article Snippet: (AA, containing penicillin, streptomycin, and amphotericin B; Invitrogen, Carlsbad, CA) at 37 °C in a humidified environment with 5% CO 2 . .. The medium was replaced with fresh medium and the following treatments were initiated: (1) rapamycin (50 nM; TargetMol, Boston, MA, USA) for 3 h, 6 h, 12 h, and 24 h; (2) pretreatment with TG (Thapsigargin; 50 nM; an ERS activator; TargetMol) or 4-PBA (4-Phenylbutyric acid; 1 mM, an ERS inhibitor; Abcam, Cambridge, UK) or SC79 (10 µM; a PI3K/AKT pathway activator; TargetMol) or LY294002 (10 µM, a PI3K/AKT pathway inhibitor; TargetMol) for 2 h, followed by rapamycin treatment.



    Similar Products

    94
    TargetMol pretreatment with tg
    Pretreatment With Tg, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pretreatment+with+tg/Thapsigargin/pm39966508-51-32-41
    Average 94 stars, based on 1 article reviews
    pretreatment with tg - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results